Evaluation of a Chromogenic Methicillin Resistant Staphylococcus Aureus Selective Medium

Authors

1 M.B.B.Ch, Demonstrator of Medical Microbiology and Immunology, Faculty of medicine (Assuit), Al Azhar University.

2 Professor of Medical Microbiology and Immunology, Faculty of Medicine, Al Azhar University

3 Assistant Professor of Medical Microbiology and Immunology, Faculty of Medicine (Assuit), Al Azhar University

4 Lecturer of Medical Microbiology and Immunology, Faculty of Medicine, Al Azhar University

Abstract

Background: One of the main sources of acquired bacterial infections in the community and hospitals is Staphylococcus aureus, particularly MRSA strains. Aim of the work: To compare between the CHROMagarTM MRSA selective medium (as a rapid method) and the other laboratory conventional methods. Also, to evaluate of the presence of mecA gene within MRSA isolates using Polymerase chain reaction (PCR). Materials and Methods: The samples were screened for antibiotic susceptibility testing and growth on a particular chromogenic medium for the detection of MRSA and their resistance rates to other routinely used antibiotics. Additionally, gel-based PCR technique was used for the detection of mecA gene. Results: Of the subjects under investigation, 200 (26.7%) had Staphylococcus aureus isolates. MRSA was detected in 86 cases (43%). MRSA was mostly isolated from newly admitted patients (75,6%) and medical personnel (24,4%). The clinical samples with the greatest MRSA prevalence (43.3%) were pus swabs. In 97.6% of cases, chromogenic agar was successfully identified MRSA isolates. All isolates that were resistant to oxacillin disc had positive PCR results. Conclusion: Using of chromogenic media as a rapid screening method and accurate diagnosis of MRSA strains is depended upon its high sensitivity and specificity compared to PCR method.

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