Prevalence of bacteriuria, candiduria and antibiotics susceptibility patterns among diabetic verses nondiabetic patients

El--Sherbiny, Gamal Mohamed; Elsaeed, Abdallah Mohamed; Radwan, Ahamed Ali; Mohamed, Mostafa Kamal Eldin

doi:10.21608/aimj.2020.38649.1297

Prevalence of bacteriuria, candiduria and antibiotics susceptibility patterns among diabetic verses nondiabetic patients

Document Type : Original Article

Authors

¹ Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University Cairo, Egypt

² Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University Cairo, Egypt.

³ Botany and Microbiology Department, Faculty Science, Al-Azhar University, Cairo, Egypt

⁴ Clinical Pathology Department, Faculty Medicine Helwan University Cairo ,Egypt

10.21608/aimj.2020.38649.1297

Abstract

Aimed of the work; Assessment of the microbial species from diabetic and nondiabetic illness and their antibiotics susceptibility patterns. Patients and Methods. Two hundred urine specimens were collected from illness in a duration period extending from July 2018 to July 20119 at Badr University Hospital-Helwan, Cairo, Egypt. Samples were collecting cultivated on MacConkey, blood agar, and Sabouraud dextrose media. The microbial isolates were identified by microbiological methods. The antibiotic sensitivity was evaluated by the VITEK 2 compact automatic system and disk diffusion method.
Results: Only 120 samples exhibit growth with a prevalence rate of 60%, from total samples collected. The results revealed UTIs were found to be significantly higher in diabetic illness (79%) compared to nondiabetic illness (41%). One hundred and twenty microbial species were recovered from collected urine specimens (79 from diabetic and 41 nondiabetics). Pathogenic bacteria & Candida were represented with 96 isolates (80.0 %) and 24 isolates (20.0), respectively. Among the 96 bacterial isolates, Escherichia coli constituted, Escherichia coli (72.80%), Klebsiella pneumonia (22.88%), and Staphylococcus aureus (4.16%). Meropenem, imipenem, trimethoprim/ sulphamethoxazole, and norfloxacin highly effective antibiotics against E. coli and Klebsiella pneumonia while amoxicillin, erythromycin, and vancomycin low effective.
Conclusion: Diabetes disease a remarkable factor that stimulates UTIs. Higher frequency resistance to antibiotics in this study renders its indecency for empirical treatment and development of new empirical treatment.

Keywords